SrasV1, Main, Exploration, bibRecord, 003508

Determination and application of immunodominant regions of SARS coronavirus spike and nucleocapsid proteins recognized by sera from different animal species

Identifieur interne : 003508 ( Main/Exploration ); précédent : 003507; suivant : 003509

Determination and application of immunodominant regions of SARS coronavirus spike and nucleocapsid proteins recognized by sera from different animal species

Auteurs : MENG YU [Australie] ; Vicky Stevens [Australie] ; Jody D. Berry [Canada] ; Gary Crameri [Australie] ; Jennifer Mceachern [Australie] ; CHANGCHUN TU [République populaire de Chine] ; ZHENGLI SHI [République populaire de Chine] ; GUODONG LIANG [République populaire de Chine] ; Hana Weingartl [Canada] ; Jane Cardosa [Malaisie] ; Bryan T. Eaton [Australie] ; Lin-Fa Wang [Australie]

Source :

Journal of immunological methods [ 0022-1759 ] ; 2008.

RBID : Pascal:08-0157693

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Immunodominance, Nucléocapside, Protéine, Anticorps, Technique ELISA, Méthode immunologique.

English descriptors

KwdEn :
- Antibody, ELISA assay, Immunodominance, Immunological method, Nucleocapsid, Protein, Severe acute respiratory syndrome virus.

Abstract

Knowledge of immunodominant regions in major viral antigens is important for rational design of effective vaccines and diagnostic tests. Although there have been many reports of such work done for SARS-CoV, these were mainly focused on the immune responses of humans and mice. In this study, we aim to search for and compare immunodominant regions of the spike (S) and nucleocapsid (N) proteins which are recognized by sera from different animal species, including mouse, rat, rabbit, civet, pig and horse. Twelve overlapping recombinant protein fragments were produced in Escherichia coli, six each for the S and N proteins, which covered the entire coding region of the two proteins. Using a membrane-strip based Western blot approach, the reactivity of each antigen fragment against a panel of animal sera was determined. Immunodominant regions containing linear epitopes, which reacted with sera from all the species tested, were identified for both proteins. The S3 fragment (aa 402-622) and the N4 fragment (aa 220-336) were the most immunodominant among the six S and N fragments, respectively. Antibodies raised against the S3 fragment were able to block the binding of a panel of S-specific monoclonal antibodies (mAb) to SARS-CoV in ELISA, further demonstrating the immunodominance of this region. Based on these findings, one-step competition ELISAs were established which were able to detect SARS-CoV antibodies from human and at least seven different animal species. Considering that a large number of animal species are known to be susceptible to SARS-CoV, these assays will be a useful tool to trace the origin and transmission of SARS-CoV and to minimise the risk of animal-to-human transmission.

Affiliations:

Australie, Canada, Malaisie, République populaire de Chine
Dongbei, Hubei, Jilin
Changchun, Pékin, Wuhan

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000307
to stream PascalFrancis, to step Curation: 000681
to stream PascalFrancis, to step Checkpoint: 000288
to stream Main, to step Merge: 003614
to stream Main, to step Curation: 003508

Le document en format XML

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<term>Severe acute respiratory syndrome virus</term>
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<front><div type="abstract" xml:lang="en">Knowledge of immunodominant regions in major viral antigens is important for rational design of effective vaccines and diagnostic tests. Although there have been many reports of such work done for SARS-CoV, these were mainly focused on the immune responses of humans and mice. In this study, we aim to search for and compare immunodominant regions of the spike (S) and nucleocapsid (N) proteins which are recognized by sera from different animal species, including mouse, rat, rabbit, civet, pig and horse. Twelve overlapping recombinant protein fragments were produced in Escherichia coli, six each for the S and N proteins, which covered the entire coding region of the two proteins. Using a membrane-strip based Western blot approach, the reactivity of each antigen fragment against a panel of animal sera was determined. Immunodominant regions containing linear epitopes, which reacted with sera from all the species tested, were identified for both proteins. The S3 fragment (aa 402-622) and the N4 fragment (aa 220-336) were the most immunodominant among the six S and N fragments, respectively. Antibodies raised against the S3 fragment were able to block the binding of a panel of S-specific monoclonal antibodies (mAb) to SARS-CoV in ELISA, further demonstrating the immunodominance of this region. Based on these findings, one-step competition ELISAs were established which were able to detect SARS-CoV antibodies from human and at least seven different animal species. Considering that a large number of animal species are known to be susceptible to SARS-CoV, these assays will be a useful tool to trace the origin and transmission of SARS-CoV and to minimise the risk of animal-to-human transmission.</div>
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   |texte=   Determination and application of immunodominant regions of SARS coronavirus spike and nucleocapsid proteins recognized by sera from different animal species
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Determination and application of immunodominant regions of SARS coronavirus spike and nucleocapsid proteins recognized by sera from different animal species

Determination and application of immunodominant regions of SARS coronavirus spike and nucleocapsid proteins recognized by sera from different animal species

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